Iodine-125 Seeds Inhibit Carcinogenesis of Hepatocellular Carcinoma Cells by Suppressing Epithelial-Mesenchymal Transition via TGF-β1/Smad Signaling

Figure 3

I-125 seed irradiation inhibits EMT of PLC and Huh7 cells. (a) Morphological changes of PLC (upper panel) and Huh7 (lower panel) cells after irradiation (red arrow indicates the EMT site). (b) By immunofluorescence staining, I-125 seed irradiation upregulated the expression of E-cadherin in PLC (left) and Huh7 (right) cells. (c) RT-PCR assay demonstrates that I-125 seed radiation upregulated the expression of E-cadherin but downregulate that of N-cadherin and vimentin. (d, e) WB assay indicated that I-125 seed radiation increase the expression of E-cadherin and weak that of N-cadherin and vimentin. The expression of data as the (). , vs. the control group.  μm.

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