Iodine-125 Seeds Inhibit Carcinogenesis of Hepatocellular Carcinoma Cells by Suppressing Epithelial-Mesenchymal Transition via TGF-β1/Smad Signaling

Table 1

Sequences of PCR primers for target gene detection.

Gene	Sequence (5->3)	Length (bp)	Tm^1 (°C)	Product size (bp)
E-cadherin-F	ATTTTTCCCTCGACACCCGAT	21	61.5	109
E-cadherin-R	TCCCAGGCGTAGACCAAGA	19	61.9
N-cadherin-F	TGCGGTACAGTGTAACTGGG	20	61.5	123
N-cadherin-R	GAAACCGGGCTATCTGCTCG	20	62.7
Vimentin-F	GACGCCATCAACACCGAGTT	21	62.4	98
Vimentin-R	CTTTGTCGTTGGTTAGCTGGT	21	62.5
TGF-β1-F	CAATTCCTGGCGATACCTCAG	21	60.2	86
TGF-β1-R	GCACAACTCCGGTGACATCAA	21	62.9
Smad2-F	CCGACACACCGAGATCCTAAC	21	61.9	125
Smad2-R	GAGGTGGCGTTTCTGGAATATAA	23	60.1
Snail-F	TCGGAAGCCTAACTACAGCGA	21	62.7	140
Snail-R	AGATGAGCATTGGCAGCGAG	20	62.6

Internal standard: GAPDH, F: TCAAGAAGGTGGTGAAGCAGG, R: AGCGTCAAAGGTGGAGGAGTG. [1]Tm: temperature.