lncRNA DLEU2 Accelerates Oral Cancer Progression via miR-30a-5p/RAP1B Axis to Regulate p38 MAPK Signaling Pathway

Figure 3

RAP1B targets miR-30a-5p and reverses the effects of si-DLEU2 in Tca8113 and CAL-27 cells. (a) Scatter plot shows differentially expressed mRNAs between the oral cancer and control groups. (b) KEGG pathway enrichment analysis. (c) The key mRNAs affecting the MAPK signaling pathway were combined with putative target genes of miR-30a-5p in the starBase database to screen for suitable mRNAs. (d) Relative expression of DLEU2 in RWPE-2 and four oral cancer cell lines (C4-2, 22RV1, Tca8113, and CAL-27). (e) The TargetScan database predicts the binding sites of miR-30a-5p and RAP1B. (f) Dual-luciferase assay analysis of the binding of RAP1B to miR-30a-5p. (g) RT-qPCR analysis of the effect of si-DLEU2 on the expression of RAP1B in Tca8113 and CAL-27 cells. (h) RT-qPCR verified the validity of overexpressing RAP1B plasmid. (i) Western blot verified the validity of overexpressing RAP1B plasmid. (j) RT-qPCR analysis of the reversal of DLEU2 expression by overexpressing RAP1B on si-DLEU2. .

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