Qingfei Tongluo Formula Mitigates Mycoplasma pneumoniae Infection via the PERK Signaling Pathway

Figure 2

QTF alleviates MP infection-induced cytokine release, ROS production, and ER stress in A549 cells. (a) The TNF-α and IL-8 release. (b) Western blot assay was used to detect the cytoplasmic and nuclear NF-κB protein levels. (c) Flow cytometry was used to measure ROS production. (d) Real-time PCR was used to measure PERK and ATF6 mRNA levels. (e) The PERK, eIF2α, p-eIF2α, ATF4, and CHOP protein levels were measured. (f) TUNEL assay was performed to detect apoptotic cells. ×200. (g) Immunofluorescence was used to measure caspase 3, CHOP, and PERK levels. ×200. vs. control; #, ##, and ### vs. vehicle; +, ++, and +++ vs. 0.1 mg/mL; $, $$ vs 0.2 mg/mL.

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