Qingfei Tongluo Formula Mitigates Mycoplasma pneumoniae Infection via the PERK Signaling Pathway

Figure 3

PERK knockdown alleviates MP infection-induced cytokine release, ROS production, and ER stress in A549 cells. (a) The PERK protein level was detected after shPERK lentivirus transduction. (b) TNF-α and IL-8 release. (c) Western blot assay was used to detect the cytoplasmic and nuclear NF-κB protein levels. (d) ROS production was measured by flow cytometry. (e) The PERK, eIF2α, p-eIF2α, ATF4, and CHOP protein levels were measured. (f) TUNEL assay was performed to detect apoptotic cells. ×200. (g) The caspase 3, CHOP, and PERK levels were measured using immunofluorescence. ×200. , vs. control; #, ##, and ### vs. MP-shNC.

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