lncRNA-DANCR Promotes Taxol Resistance of Prostate Cancer Cells through Modulating the miR-33b-5p-LDHA Axis

Figure 7

miR-33b-5p targets 3UTR of LDHA in PCa cells. (a) Prediction of binding sites of miR-33b-5p on LDHA 3UTR. (b) Expressions of LDHA from prostate tumors and normal prostate tissues were analyzed by http://ualcan.path.uab.edu from TCGA database. (c) Expressions of miR-LDHA from prostate tumors () and adjacent normal tissues () were analyzed by qRT-PCR. (d) Expressions of LDHA were examined in two non-tumorous prostate cell lines and four PCa cell lines. (e) Expressions of LDHA were examined in parental and Taxol-resistant PC3 cells. (f) Correlation analysis of LDHA and miR-33b-5p in prostate tumor specimens. (g and h) PC3 and LN96 cells were transfected with control miRNA or miR-33b-5b. Cells were treated with Taxol, and cell viability was determined by MTT assay. (i) Protein expressions of LDHA were examined from the above transfected cells. (j and k) Luciferase assay was performed in PC3 and LN96 cells with cotransfection of control miRNA or miR-33b-5p plus luciferase vector containing WT- or Mut-LDHA 3UTR. , , and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)