Death-Associated Protein Kinase 1 (DAPK1) Protects against Myocardial Injury Induced by Myocardial Infarction in Rats via Inhibition of Inflammation and Oxidative Stress

Figure 4

Inhibition of DAPK1 alleviates IL-1β-induced H9c2 cell injury. Expression of DAPK1 in H9c2 cells was detected by IF staining (magnification: 200x) (a) and RT-PCR (b). CCK8 (c) detected the effect of 1, 5, 10, 20, and 50 μM TC DAPK 6 on H9c2 cells viability. The activities of MDA (d), GSH (e), SOD (f), and CAT (g) were measured to determine the level of oxidative stress in H9c2 cells. ROS level in H9c2 cells was detected by flow cytometry (h). The mRNA expression of Prdx1 (i), Prdx4 (j), GPX1 (k), and GPX3 (l) was detected by RT-PCR (“” means vs. control group, and “^#” means vs. IL-1β group).

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