ATF3 Regulates Osteogenic Function by Mediating Osteoblast Ferroptosis in Type 2 Diabetic Osteoporosis

Figure 1

Ferroptosis is induced by HG in hFob1.19 cells. (a) Cells were treated with HG for 24 h, 48 h, 72 h, and 96 h. Cell viability was measured using the CCK-8 assay, and the data were expressed as values relative to the NG group. (b) The LDH enzyme activity was quantified using the LDH content kit. (c) Transmission electron microscopy images of hFob1.19 cells treated with HG and NG. Ferroptosis was only observed in the HG group. (d) HG caused an increased level of PTGS2 mRNA. (e) Intracellular iron ions were detected using FeRhoNox-1 fluorescent probe. Scale  μm. (f) hFob1.19 cells were treated with NG and HG, respectively. The protein levels of ferroptosis-related protein GPX4 and ATF3 were determined by western blot. All data are presented as the of three independent experiments. vs. NG and vs. NG.

(a)

(b)

(c)

(d)

(e)

(f)