Research Article

[Retracted] Activated Hepatic Stellate Cells Promote the M1 to M2 Macrophage Transformation and Liver Fibrosis by Elevating the Histone Acetylation Level

Figure 3

Regulation of primary activated HSCs on the M1/M2 phenotypic transition of macrophages. (a) Western blot showing expression of α-SMA in the HSC and a-HSC (activated HSCs). (b) Lactate level in HSCs and a-HSCs. (c) The level of glycolysis in HSCs and a-HSCs. (d) Detection of the expression levels of M1 macrophage surface markers IL-1β, IL-6, iNOS, and TNF-α in co-aLX-2 co-LX-a cells and their supernatants by ELISA. (e) Detection of the expression levels of M2 macrophage surface markers Arg1, CD163, IL-10, and TGFβ in the supernatants of HSCs and a-HSCs by ELISA. (f) Western blot shows the expression of histone acetylation in the supernatants of HSCs and a-HSCs. , , and .
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