hsa_circ_0139402 Promotes Bladder Cancer Progression by Regulating hsa-miR-326/PAX8 Signaling

Figure 4

hsa_circ_0139402 inhibits bladder cells’ proliferation, migration, invasion, and EMT by targeting PAX8 via hsa-miR-326. (a) CCK8 assay showed that negative control or sh-hsa_circ_0139402 or sh-hsa_circ_0139402+hsa-miR-326 inhibition or hsa-miR-326 inhibition could regulate 5637 and UMUC3 cells’ proliferation; (b) wound healing assay showed that negative control or sh-hsa_circ_0139402 or sh-hsa_circ_0139402+hsa-miR-326 inhibition or hsa-miR-326 inhibition could regulate 5637 and UMUC3 cells’ migration; (c) transwell assay showed that negative control or sh-hsa_circ_0139402 or sh-hsa_circ_0139402+hsa-miR-326 inhibition or hsa-miR-326 inhibition could regulate 5637 and UMUC3 cells’ invasion; (d–f) western blot analysis showed that the expression levels of PAX8, E-cadherin, N-cadherin, and Vimentin transfected with sh-hsa_circ_0139402+hsa-miR-326 inhibition or hsa-miR-326 inhibition could regulate 5637 and UMUC3 cells. Data were represented as . ; ^# compared with sh-circPVT1+miR-140-3p inhibition.

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