Label-Free Proteomics of Oral Mucosa Tissue to Identify Potential Biomarkers That Can Flag Predilection of Precancerous Lesions to Oral Cell Carcinoma: A Preliminary Study

Figure 1

Label-free quantification proteomic analysis workflow. Sixteen biological replicates with suspected lesions were recruited. Disease phenotypes, control, leukoplakia, erythroplakia, and cancer, were assigned after clinical and histopathological evaluations. Oral mucosal tissues were processed for protein isolation, trypsin digestion, and desalting. Peptides were subjected to mass spectrometric analysis, and the generated raw files were analyzed for protein identification. Results were validated via bioinformatic analysis.