Aldolase A Promotes Colorectal Cancer Progression through Targeting COPS6 and Regulating MAPK Signaling Pathway

Figure 5

Protein-protein binding relationship between ALDOA and COPS6. (a) In stable overexpressed ALDOA cell lines tagged with flag, utilizing the characteristic of specific binding to flag beads, IP assays were performed to identify the interactive proteins that could bind to ALDOA. (b) Fifteen protein molecules and fragments were found to have the potential to bind to ALDOA according to the two MS analyses. (c–e) IP assays and western blotting were performed to confirm the binding relationship between ALDOA and COPS6.

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