Silencing MYOT Expression May Inhibit Autophagy in Human Skeletal Muscle Cells

Figure 1

The MYOT was knocked down effectively by siRNA in HSkMCs. (a) Real-time PCR detected the mRNA levels of the MYOT in HSkMCs transfected with three siRNAs, respectively. (b) Real-time PCR detected the mRNA levels of the MYOT in HSkMCs transfected with different concentrations of si-MYOT(3). (c) Western blot detected the protein levels of MYOT in HSkMCs transfected with 30 nm si-MYOT(3). (d) The statistical results of MYOT. (e) CCK8 detected the cell viability in the WT, si-control, and si-MYOT groups.

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