Endothelial Progenitor Cells Affect the Growth and Apoptosis of Renal Cells by Secreting Microvesicles Carrying Dysregulated miR-205 and miR-206

Figure 2

EPCs carry dysregulated miR-205 and miR-206 through the secreted MV pathway. (a) Dil-Ac-LDL staining was used to analyze the separation of EPCs. Blue is DAPI staining, and red is Dil-Ac-LDL staining. (b) TEM analysis of the extraction of MVs. Magnification: 40,000x. (c) Western blot analysis of the protein expression of CD63, CD81, and Tsg101 in the extracted MVs. (d–i) RT-qPCR analysis of the expression changes of miR-205 and miR-206 in EPCs (d, e), supernatants (f, g), and MVs (h, i). . Dil-Ac-LDL: Dil complex acetylated low-density lipoprotein; DAPI: 4,6-diamidino-2-phenylindole; EPCs: endothelial progenitor cells; MVs: microvesicles; RT-qPCR: real-time quantitative polymerase chain reaction; TEM: transmission electron microscopy; IF: immunofluorescence; miR: microRNA.

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