Endothelial Progenitor Cells Affect the Growth and Apoptosis of Renal Cells by Secreting Microvesicles Carrying Dysregulated miR-205 and miR-206

Figure 4

miR-205 and miR-206-MVs protect PRKs from Ang II damage in vivo. (a) IF analysis of the expression intensity of vimentin and α-SMA in the isolated PRKs. Blue is DAPI, green is anti-vimentin antibody, and red is anti-α-SMA antibody. (b) Fluorescence microscopy was used to observe the fusion of PKH26-labeled EPC-MVs and PRKs. Blue is DAPI, and red is MVs carrying PKH26-labeled. (c, d) RT-qPCR analysis in vitro miR-205 and miR-206-MVs regulate the expression of miR-205 and miR-206 in PRKs. (e, f) CCK8 analysis of the effects of miR-205 and miR-206-MVs on the proliferation of PRKs induced by Ang II in vitro. . RT-qPCR: real-time quantitative polymerase chain reaction; miR: microRNA; CCK-8: Cell Counting Kit-8; Ang II: angiotensin II; EPCs: endothelial progenitor cells; PRKs: primary rat kidney cells; MVs: microvesicles; IF: immunofluorescence; α-SMA: α-smooth muscle actin.

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