Endothelial Progenitor Cells Affect the Growth and Apoptosis of Renal Cells by Secreting Microvesicles Carrying Dysregulated miR-205 and miR-206

Figure 5

The mechanism by which miR-205 and miR-206 work is achieved by targeting downstream DDX5. (a) The miRDB database analyzed the common downstream targets of miR-205 and miR-206. (b, c) RT-qPCR analysis in vitro miR-205 (b) and miR-206 (c) MVs regulate DDX5 expression. (d, e) RT-qPCR analysis in vivo miR-205 (d) and miR-206 (e) MVs regulate DDX5 expression. (f) RT-qPCR verified the validity of the overexpressed DDX5 plasmid. (g) Western blot verified the effectiveness of overexpressing DDX5 plasmid. (h) RT-qPCR analysis of the effect of overexpressing DDX5 in the presence of miR-205 or miR-206 agomiR on DDX5 expression. (i) RT-qPCR analysis of the effect of overexpressing DDX5 in the presence of miR-205 or miR-206 agomiR on miR-205 or miR-206 expression. (j, k) The miRDB database analysis of the binding sites of miR-205 (j) or miR-206 (k) to DDX5. (l, m) Dual-luciferase assays for direct binding of miR-205 (l) or miR-206 (m) to DDX5. . RT-qPCR; real-time quantitative polymerase chain reaction; miR: microRNA; ov: overexpression; DDX5: DEAD-box helicase 5; EPCs: endothelial progenitor cells; PRKs: primary rat kidney cells; MVs: microvesicles; WT: wild type; mut: mutant.

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