YB-1 Expression Is Associated with Lymph Node Metastasis and Drug Resistance to Adriamycin in Breast Cancer

Figure 2

Proliferation, cell cycle progression, invasion, and migration were evaluated in MCF-7/ADR cells. (a) The ADR concentration used to establish the ADR-resistant cell line was determined by MTT assay and resistance of MCF-7/ADR to other drugs (CDDP and 5-FU). (b) Tumor spheroid formation (magnification 40x) and (c) cell morphology (magnification 100x). (d) Cell proliferation was detected by CCK-8 in 24 h, 48 h, and 72 h. (e) Cell cycle progression was examined by flow cytometry. (f) Cell invasion and migration were assayed by Transwell test (magnification 200x). Value was presented for three times to conduct statistical analysis. (g) The weight and size (long and short diameter) of tumors formed in the mice. (h) Comparison of YB-1, BCRP, cyclin E, cyclin D1, p53, and GAPDH expressions in different groups by Western blot. Value was presented for three times to conduct statistical analysis. In vivo experiments , in vitro experiment , means , means , and means .

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