TRIM58 Interacts with ZEB1 to Suppress NSCLC Tumor Malignancy by Promoting ZEB1 Protein Degradation via UPP

Figure 1

Significant TRIM58 downregulation in NSCLC was observed. (a) Differential expression of TRIM58 protein in NSLCL tumor tissue and normal lung tissue was analyzed with IHC staining. (b) H-Score comparison was performed on clinical samples of NSCLC patients retrospectively collected in our clinical center. (c) Western blot study on TRIM58 protein expression among four different NSCLC tumor tissues and adjacent normal lung tissue pairs. (d) qRT-PCR quantification of TRIM58 mRNA level between NSCLC tumor tissue and normal lung tissues. Samples were retrospectively collected in NSCLC patients receiving surgery in our clinical center. (e) Distribution of TRIM58 mRNA expression strength illustrated as Log2 value of expression ratio of tumor and matched normal lung tissue pairs retrospectively collected in our clinical center. (f) Overall survival comparison between NSCLC patient groups with high or low TRIM58 expression level. Log-rank test was utilized to examine the statistical significance. (g) TRIM58 expression among normal lung epithelial cell line BEAS-2B and NSCLC cancer cell lines including NCI-H441/NCI-H157/NCI-H1975/NCI-H1395/NCI-H2087 was measured with western blot. (h) qRT-PCR analysis was performed to measure the mRNA expression of TRIM58.

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