TRIM58 Interacts with ZEB1 to Suppress NSCLC Tumor Malignancy by Promoting ZEB1 Protein Degradation via UPP

Figure 3

Association of TRIM58 with NSCLC chemoresistance and stem-like cellular phenotype. (a–c) After transfection with TRIM58 specific shRNAs or vector, the chemoresistance of NCI-H1395 cell lines treated with various concentrations of docetaxel, doxorubicin, or gefitinib were evaluated. (d–f) After transfection with TRIM58 overexpression vectors or control vector, the chemoresistance of NCI-H441 cell lines treated with various concentrations of docetaxel, doxorubicin, or gefitinib were measured. (g, h) Tumor stem-like cell marker detection via western blot/qRT-PCR methods, including ALDH1 and CD44. Cell line groups detected were treated with TRIM58 specific shRNAs/overexpression vectors/negative control vectors.

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