TRIM58 Interacts with ZEB1 to Suppress NSCLC Tumor Malignancy by Promoting ZEB1 Protein Degradation via UPP

Figure 5

Degradation of ZEB1 was promoted by TRIM58 expression. (a, b) CHX chase experiment was conducted to measure UPP inhibition impact on TRIM58 and ZEB1 expression in NCI-H1395 cell line after treatment with TRIM58 overexpression vector or control vector. (c, d) CHX chase experiment was conducted to measure UPP inhibition impact on TRIM58 and ZEB1 expression in NCI-H441 cell line after treatment with TRIM58 shRNAs or negative control vector. (e, f) Co-IP was performed to detect the effects of TRIM58 modulation on ZEB1 protein expression in NSCLC cell lines. All cell groups were treated with MG132 to inhibit UPP, and each experimental group was additionally treated by negative control vector/TRIM58 shRNAs/TRIM58 overexpression vectors.

(a)

(b)

(c)

(d)

(e)

(f)