hsa_circ_0129047 Upregulates LYVE1 to Inhibit Hepatocellular Carcinoma Progression by Sponging miR-492

Figure 3

Circ_0129047 targets miR-492. (a) The binding pairs of miR-492 and circ_0129047 were predicted by CircInteractome. (b) Hep 3B and Huh7 cells were cotransfected with a miR-492 mimic or mimic NC and circ_0129047-WT or circ_0129047-MUT luciferase vectors. Luciferase activities were determined using the dual-luciferase system. , vs. miR-NC. (c) RT-qPCR analysis of immunoprecipitated circ_0129047 and miR-492 using an anti-Ago2 antibody. , vs. Anti-lgG. (d) RT-qPCR analysis of miR-492 expression in HCC tissues. (e) RT-qPCR analysis of miR-492 expression in HCC (Hep 3B and Huh7) and THLE2 cells. , vs. THLE2. (f) Pearson analysis of the correlation between miR-492 and circ_0129047. (g) Hep 3B and Huh7 cells transfected with OE-NC, OE-circ (OE-circ_0129047), miR-492 mimic, mimic NC, and OE-circ + mimic. The expression of miR-492 was determined by RT-qPCR at 48 hr. , vs. OE-NC; ^##, vs.mimic-NC; ^&&, vs.OE + mimic.

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