hsa_circ_0129047 Upregulates LYVE1 to Inhibit Hepatocellular Carcinoma Progression by Sponging miR-492

Figure 5

MiR-492 targets LYVE1. (a) TargetScan was used to identify the target of miR-492. (b) Luciferase activity driven by 3′UTR LYVE1 MUT and WT was assessed in Hep 3B and Huh7 cells cotransfected with miR-492 mimic or mimic NC. , vs. miR-NC. (c) RT-qPCR analysis of LYVE1 expression in HCC and normal tissues. (d) RT-qPCR analysis of LYVE1 in THLE2 and HCC cells. , vs. THLE2. (e) Pearson analysis of the association between LYVE1 and miR-492 in HCC tissues. (f) Western blotting was conducted to examine the LYVE1 expression in Hep 3B and Huh7 cells transfected with OE-NC, mimic NC, OE-circ mimic, or OE-circ + mimic. , vs. OE-NC; ^##, vs.mimic-NC; ^&&, vs.OE- circ + mimic.

(a)

(b)

(c)

(d)

(e)

(f)