Tanshinone IIA Inhibits Triple-Negative Breast Cancer Cells MDA-MB-231 via G Protein-Coupled Estrogen Receptor- (GPER-) Dependent Signaling Pathway

Figure 3

The effects of Tan IIA on cell apoptosis. (a, b) The apoptosis rate of MDA-MB-231 cells treated with 0, 5, 10, G15+10, and 50 μΜ Tan IIA for 48 h was detected by flow cytometry after staining with Annexin V-FITC/PI. coordinate represents Annexin-FITC signal, and coordinate represents PI signal. The third quadrant is normal cells, the fourth quadrant is early apoptotic cells, the first quadrant is intermediate to late apoptotic, and the second quadrant is necrotic cells. (c, d) Effect of Tan IIA on the expression of apoptosis protein caspase-3 in MDA-MB-231 cells. compared with control, ^## compared with 10 μM Tan IIA. All results were presented as means of three independent . Each independent experiment was repeated three times.

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