Tanshinone IIA Inhibits Triple-Negative Breast Cancer Cells MDA-MB-231 via G Protein-Coupled Estrogen Receptor- (GPER-) Dependent Signaling Pathway

Figure 6

The effects of Tan IIA on c-Jun and c-Fos nuclear translocation. Nuclear and cytosolic proteins were extracted in MDA-MB-231 cells to explore the nuclear transport changes of c-Fos and c-Jun through Western blot technique after, respectively, treated with 0, 5, 10, or 50 μM Tan IIA for 48 h. (a, b) Representative images of Western blotting for c-Fos and c-Jun in the nuclear and cytosolic extracts, respectively. (c–f) The quantification of the two proteins. Data were expressed as of three independent experiments. and compared with the control.

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