Tanshinone IIA Inhibits Triple-Negative Breast Cancer Cells MDA-MB-231 via G Protein-Coupled Estrogen Receptor- (GPER-) Dependent Signaling Pathway

Figure 7

Effects of Tan IIA on the activity and expression levels of cell cycle-associated proteins. (a) MDA-MB-231 cells were treated with 0, 5, 10, or 50 μM Tan IIA for 48 h, respectively. Next, Western blot was used to detect changes in the expression levels of cell cycle-associated key proteins. The band images of cyclin A2, cyclin D1, CDK2, CDK4, CDK6, and GAPDH proteins were captured via a special exposure machine. (b–f) Statistical analysis results of protein expression levels of cyclin A2, cyclin D1, CDK2, CDK4, and CDK6. Charts revealed that the protein expression of cyclin A2, cyclin D1, CDK2, CDK4, and CDK6 decreased significantly with the increase of Tan IIA concentration. Data were expressed as of three independent experiments. and compared with control.

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