Examining the Association of Rare Allelic Variants in Urate Transporters SLC22A11, SLC22A13, and SLC17A1 with Hyperuricemia and Gout

Figure 3

(a) Expression of OAT10 (SLC22A13) and its allelic variants in HEK293A cells line. Wild-type protein was tagged with a C-terminal tGFP tag, and cells were transiently transfected by polyethyleneimine lipofection. Samples were fixed with 4% paraformaldehyde, and pictures were taken with a Leica DM6 microscope at 400x magnification. Plasma membrane localization was observed in all variants. Cell nuclei were stained with DAPI (blue). (b) Western blot of OAT10 allelic variants. Expression of the protein was detected with tGFP antibody and anti-β-actin antibody as a loading control. (c) Predicted structure of OAT4 protein visualized in Protter [58]. Allelic variants are marked with red dots. (d) Uptake study with 30 µM 14C uric acid with 5-min incubation. The intracellular uptake of urate is expressed as a % of the wild-type uptake, n = 3, means (Student’s t-test).

(a)

(b)

(c)

(d)