Plasma microRNA-320c as a Potential Biomarker for the Severity of Knee Osteoarthritis and Regulates cAMP Responsive Element Binding Protein 5 (CREB5) in Chondrocytes

Figure 3

miR-320c negatively regulated CREB5 expression by targeting the CREB5 3′-untranslated region (UTR): (a) binding site for miR-320c in the CREB5 3′-UTR; (b) relative luciferase activity of CREB5 in HC-a cells (normalized to the Renilla luciferase); (c) CREB5 mRNA expression level, as determined by real-time PCR (normalized to GAPDH); and (d) CREB5 protein expression level, as determined by western blot analysis. Two-tailed student’s t test was used to compare between groups. , ^#. Wt: wild-type. Mut: mutant. Ratio: firefly luciferase activity/Renilla luciferase activity.

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