Plasma microRNA-320c as a Potential Biomarker for the Severity of Knee Osteoarthritis and Regulates cAMP Responsive Element Binding Protein 5 (CREB5) in Chondrocytes

Table 1

Primer sequences used for vector construction and real-time PCR.

Gene	Primer sequence
miR-320c	F: AAAAGCTGGGTTGAGAGGGT
	R: ACCCTCTCAACCCAGCTTTT
CREB5	F: CCCTGCCCAACCCTACAATG
	R: GGACCTTGCATCCCCATGAT
CREB5 3′-UTR Wt	F: AGTCCAACCCTTTGCCTGAAA
	R: TGACACCACAGCACAAACTCA
CREB5 3′-UTR Mut	F: CTTGGGAAACGCTTTGGTGC
	R: TAGCTGACTGTACCTGGTTTAACA
c-jun promoter	F: GCAGCGGAGCATTACCTCAT
	R: TAGCCCATGATGTCACCCCA
c-fos promoter	F: GGGGACATGCGTCTTCGC
	R: CAAGGTGCTCCAGAGTGTGC
GAPDH	F: GGAGCGAGATCCCTCCAAAAT
	R: GGCTGTTGTCATACTTCTCATGG
U6	F: GCTTCGGCAGCACATATACTAAAAT
	R: CGCTTCACGAATTTGCGTGTCAT

F: forward; R: reverse; CREB5: cAMP responsive element binding protein 5; UTR: untranslated region; Wt: wild-type; and Mut: mutant.