Circulating Nucleic Acids in Colorectal Cancer: Diagnostic and Prognostic Value
Table 1
The challenges of current semiautomated strategies for CTCs extraction and molecular quantification.
Methods
Challenges
References
The cell search system
Low sensitivity (false negative) due to only EpCAM-positive CTCs consideration or missing CTCs subclusters engaged in EMT, low specificity (false positive), small productivity, and diverse specimen testing for direct recognition and evaluation of intact cells in the epithelial cellular adhesion molecule (EpCAM)-based cell search R system
Complete separation proficiency by a single or multiple capture antibodies for pathology-specific target cells in small fluid volumes after laminar flow without pretagging
Requirement of spiked-in experiments for validation of different CTC isolation approaches prior to more accurate clinical reliability for removing interference with large leukocytes (e.g., monocytes) and CTCs due to their small size and low frequency of size-based techniques below 10%