Circulating Nucleic Acids in Colorectal Cancer: Diagnostic and Prognostic Value

Table 4

Methods for obtaining cfDNA from plasma and serum.

Methods	Approaches	References
QIAamp circulating nucleic acid kit	Easily extraction of ccfDNA with rapid; spin-column or 96-well-plate technique without dependent phenol-chloroform extraction	[75, 108]
Triton/heat/phenol protocol (THP)	Greatest efficiency, cheaper, and high-quality outputs for further plasma cfDNA isolation vs. the Qiagen kit assay	[109]
NucleoSpin® Plasma X’S	Very fast extraction of this method leading to a much integrity DNA produce that may utilize for the recovered of small DNA fragments	[110]
Maxwell® 16 LEV DNA purification kit	Automated purification of cfDNA from much less 10^4 cells using silica-coated paramagnetic particles (PMPs), as a mobile solid phase for optimizing of the capture, washing and elution of the target material	[111]