Loss of the Desmosomal Component Perp Impairs Wound Healing In Vivo
Figure 6
keratinocytes display enhanced migration capacity in vitro. (a) A mechanical dissociation assay was performed on wild-type and keratinocyte monolayers. The number of cellular fragments per 4 fields was counted and averaged. Graphs show the average of three independent experiments performed in triplicate and error bars represent +/− SEM. (b) A transwell migration assay was used to assess the migration potential of wild-type and keratinocytes. Representative image of migrated cells stained with crystal violet. (c) The number of cells migrated in each of 3 fields was counted and averaged. The results represent the average of three individual experiments +/− SEM. (d) Representative phase-contrast images of wild-type and explants. The red dashed boxes represent the area seen in (f). (e) The average areas of outgrowth after 10 days for wild-type and explants are quantified in the graph. Wild-type: . : . Error bars represent the SEM. (f) Enlarged phase-contrast images of the areas boxed in red in panel (d) showing wild-type and Perp −/− explants. Statistical significance was determined using a Student’s unpaired -test ,, .
