A Novel, Hand-Held, and Low-Level Light Therapy Device for the Treatment of Acne Vulgaris: A Single-Arm, Prospective Clinical Study

Figure 2

(a) Quantitative polymerase chain reaction analysis of skin extracellular matrix remodeling-/inflammation-related genes. The levels of gene expression of the experimental groups (low-level light therapy treatment for 20 m) were normalized to the levels of the control group (no low-level light therapy); 18 s was used as the reference gene. Values are presented as means ± standard deviation. “ns” indicates that there is no statistical difference. Histological evaluation of skin tissues from SKH1 mice with or without 20 m low-level light therapy treatment. (b) Hematoxylin and eosin staining of SKH1 skin tissues for skin extracellular matrix (pink) and nuclei (purple). (c) Ki67 staining for visualizing proliferating cells’ nuclei (dark brown) within SKH1 skin tissues. (d) Masson’s trichrome staining of SKH1 skin tissues for collagen (blue) and cytoplasm (pink). (Magnification, 10x: scale bar = 200 μm, Magnification, 20x: scale bar = 100 μm).

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