Research Article

Magnesium Compounds Increase Aquaporin-3 in Human Epidermal Keratinocyte HaCaT Cells

Figure 3

Effects of osmotic pressure on AQP3 expression. HaCaT cells were treated with magnesium acetate (10 mM; 330 mOsm or 25 mM; 375 mOsm) or sodium chloride (15 mM; 330 mOsm or 37.5 mM; 375 mOsm). After 6 hours, the mRNA expression level of AQP3 was analyzed by real-time RT-PCR. The mRNA expression levels were normalized to RPL30, and the mean value of the control was expressed as 100% (mean ± S.D., n = 4, , vs. control).