Original Article

Alpinia pricei Rhizome Extracts Induce Cell Cycle Arrest in Human Squamous Carcinoma KB Cells and Suppress Tumor Growth in Nude Mice

Figure 4

Western blot analysis of MMP-9, u-PA, u-PAR, TIMP-1 and PAI-1 protein levels in KB cells after exposure to AP extracts. (a) Cells were treated with 0, 25, 50, 100, 150 or 200 μg/mL of AP extracts for 24 h. (b) Cells were treated with 150 μg/mL of AP extracts for 0, 4, 8, 12, 18 or 24 h. Protein (50 μg) from each sample was resolved on 8–15% SDS-PAGE and western blotting was performed. β-actin was used as a control. Relative changes in protein bands were measured using densitometric analysis. Typical results from three independent experiments are shown.
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(a)
123815.fig.004b
(b)