Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage
Figure 4
mRNA and protein expressions of mesodermal and cardiac markers in hESC-derived CPs. (a) mRNA expression of cardiac-specific markers in RG-treated, hESC-derived CPs. Expressions of Brachyury, Nkx2.5, αMHC, and cardiac actin were confirmed by RT-PCR. 1 g of RNAs were used for synthesis of cDNA. (b) Protein expression of Bachyury (early stage), Nkx2.5 and cardiac troponin I (late stage) in hESC-derived CPs.