Effects of Salvianolic Acid B on Protein Expression in Human Umbilical Vein Endothelial Cells
Figure 3
Sal B treatment decreased caldesmon expression and increased vimentin and phospho-NPM expression. (a) Cell lysates (50 μg) were processed for Western blot analysis using monoclonal antibodies against TM and α-tubulin. The relative expression of caldesmon, vimentin, and NPM was quantified by densitometry and normalized to α-tubulin. Phospho-NPM was quantified by densitometry and normalized to the NPM protein. Control values were set to one. (b) Mitogenic effect of Sal B on HUVECs proliferation. HUVECs were seeded (7.5 × 104 cells/well in 96-well tissue culture plate) and pretreated with Sal B for 12 h. The cells were washed and maintained in fresh EBM containing 0.25% FBS for additional 24 h incubation at 37°C. The cell viability was measured by the metabolic activity of viable cells with the Cell Proliferation Reagent WST-1. The values are presented as means of cell count obtained from six wells for each treatment. as compared with control as determined by analysis of variance followed by an unpaired Student's -test.
