Effects of Salvianolic Acid B on Protein Expression in Human Umbilical Vein Endothelial Cells

Table 1

Identified proteins that were changed in Sal B-treated HUVEC.

Spot no.	Protein identity	SwissProt no.	Relative spot intensity % (Sal B/control, 100%)	pI/Mr	Mascot score	Cellular location	Molecular function
1	Caldesmon	Q05682	45%	5.5/93.3	81	Cytoskeleton	Actin binding, calmodulin binding, myosin binding, tropomyosin binding
2	Vimentin	P08670	227%	4.9/53.7	129	Cytoplasm intermediate filament	Protein binding, structural constituent of cytoskeleton
3	T-complex protein 1 subunit beta	P78371	Sal B only	6/57.8	223	Nucleus	ATP binding, unfolded protein binding
4	Protein disulfide-isomerase A3	P30101	260%	5.9/57.1	180	Endoplasmic reticulum melanosome	Cysteine-type endopeptidase activity, phospholipase C activity protein disulfide isomerase activity
5	Nucleophosmin	P06748	Sal B only	4.5/32.7	67	Nucleus, nucleoplasm, spindle pore cetrosome
6	Heat shock protein beta-1	P04792	230%	6/22.8	135	Cell surface, cytoplasm nucleus	Identical protein binding
7	Tropomyosin alpha-3 chain	P06753	Only Sal B	4.5/32.9	85	Muscle thin filament tropomyosin	Actin binding
8	UBX domain-containing protein 1	Q04323	316%	5.1/33.4	153	Cytoplasm	ATPase binding, polyubiquitin binding
9	Alpha-enolase	P06733	237%	7.7/47.5	127	Nucleus, plasma membrane	Magnesium ion binding, serine-type endopeptidase activity, phosphopyruvate hydratase activity, transcription factor activity
10	Peroxiredoxin-2	P32119	258%	5.6/22	140	Cytoplasm	Thioredoxin peroxidase activity