Bufalin Induces Reactive Oxygen Species Dependent Bax Translocation and Apoptosis in ASTC-a-1 Cells
Figure 5
Mitochondrial dysfunction induced by bufalin. (a) Typical fluorescent images of mitochondria under different treatments. The mitochondria in bufalin-treated cells were obviously swollen. Scale bar: 10 μm. (b) Quantification of mitochondrial width under different treatments. **P <.05, compared with control. #P <.05, compared with treatment with bufalin alone. (c) FCM analysis of mitochondrial membrane potential (ΔΨm) loss induced by bufalin. The proportions of cells with reduced Rho123 fluorescence were shown. Experiments were performed in triplicate with similar results.
