Aqueous Extract of Shi-Liu-Wei-Liu-Qi-Yin Induces G2/M Phase Arrest and Apoptosis in Human Bladder Carcinoma Cells via Fas and Mitochondrial Pathway
Figure 4
SLWLQY decreases the protein expression of G2 cell-cycle regulatory CDKs and cyclins in TSGH-8301 cells. Cells were treated with or without SLWLQY 0, 1, 3 and 5 mg ml−1 for 24 h. Total cell lysates were prepared and subjected to SDS–PAGE followed by Western blot, the membranes were probed with anti-cyclinB1 and Cdc2 antibody, and the results were represented by using an ECL system. β-actin was the loading control. Results were averaged from triplicate experiments and expressed in mean ± SD. **P < 0.01.
