Mutagenic Activity of Indigofera truxillensis and I. suffruticosa Aerial Parts
Table 1
Mutagenic activity expressed as the mean and SD of the number of revertants/plate in bacterial strains TA98, TA100, TA97a and TA102 exposed to MeOH extract and fractions A, B and C of I. truxillensis at various doses, with (+S9) or without (−S9) metabolic activation.
Treatments mg/plate
Revertants/plate in S. typhimurium strains
TA98
TA97a
TA100
TA102
−S9
+S9
−S9
+S9
−S9
+S9
−S9
+S9
MeOH extract
0
43 ± 2.7
36 ± 2.0
131 ± 1.5
139 ± 2.5
163 ± 4.2
145 ± 9.5
242 ± 9.2
223 ± 11.9
1.25
55 ± 4.6* (1.3)
41 ± 2.3 (1.1)
143 ± 5.0 (1.1)
131 ± 2.5 (0.9)
184 ± 8.7 (1.1)
151 ± 14.5 (1.0)
271 ± 4.0 (1.1)
219 ± 7.9 (0.9)
2.50
57 ± 1.5** (1.3)
38 ± 7.6 (1.1)
143 ± 3.1 (1.1)
129 ± 10.7 (0.9)
186 ± 7.6 (1.1)
145 ± 3.0 (1.0)
254 ± 1.5 (1.0)
223 ± 11.0 (1.0)
3.75
87 ± 2.0** (2.0)
42 ± 2.0 (1.3)
147 ± 2.1 (1.1)
137 ± 6.8 (0.9)
194 ± 4.0 (1.2)
149 ± 11.9 (1.0)
258 ± 1.0 (1.1)
238 ± 6.7 (1.1)
5.00
77 ± 6.1** (1.8)
45 ± 4.2 (1.3)
148 ± 5.0 (1.1)
142 ± 16.1 (1.0)
180 ± 6.2 (1.1)
141 ± 7.6 (0.9)
264 ± 4.0 (1.1)
228 ± 5.3 (1.0)
7.50
75 ± 5.0** (1.7)
51 ± 3.1(1.4)
159 ± 7.8 (1.2)
134 ± 16.0 (0.9)
173 ± 5.0 (1.1)
140 ± 5.5 (0.9)
280 ± 35.6 (1.2)
249 ± 11.0 (1.1)
Fraction A (glycerolipids)
0
29 ± 2,1
31 ± 2.7
141 ± 8.0
143 ± 4.0
148 ± 2.5
143 ± 5.0
239 ± 16.7
210 ± 11.1
0.12
38 ± 4.6 (1.3)
31 ± 3.6 (1.0)
143 ± 13.5 (1.0)
137 ± 5.3 (0.9)
163 ± 26.1 (1.1)
155 ± 4.2 (1.1)
267 ± 14.1 (1.1)
235 ± 13.1 (1.1)
0.38
32 ± 5.3 (1.1)
30 ± 1.5 (0.9)
138 ± 4.6 (0.9)
143 ± 4.0 (1.0)
151 ± 4.2 (1.0)
149 ± 7.0 (1.0)
247 ± 7.6 (1.0)
236 ± 10.3 (1.1)
0.75
42 ± 13.5 (1.4)
40 ± 4.5 (1.3)
132 ± 7.8 (0.9)
148 ± 3.6 (1.0)
164 ± 13.9 (1.1)
165 ± 13.6 (1.2)
245 ± 5.9 (1.0)
251 ± 5.1 (1.2)
1.50
39 ± 5.6 (1.3)
35 ± 3.6 (1.1)
144 ± 8.4 (1.0)
138 ± 7.6 (0.9)
183 ± 14.1 (1.2)
166 ± 3.1 (1.2)
250 ± 6.1 (1.0)
254 ± 5.6 (1.2)
2.50
40 ± 4.1 (1.4)
39 ± 6.0 (1.3)
138 ± 4.6 (0.9)
143 ± 6.1 (1.0)
173 ± 10.1 (1.2)
175 ± 7.6 (1.2)
251 ± 6.9 (1.0)
255 ± 7.2 (1.2)
Fraction B (flavonoids)
0
29 ± 2,1
31 ± 2.7
141 ± 8.0
143 ± 4.0
148 ± 2.5
143 ± 5.0
239 ± 16.7
210 ± 11.1
0.12
32 ± 4.0 (1.1)
30 ± 5.9 (0.9)
142 ± 20.0 (1.0)
145 ± 7.9 (1.0)
144 ± 14.2 (0.9)
145 ± 4.2 (1.0)
223 ± 11.0 (0.9)
224 ± 6.0 (1.1)
0.38
40 ± 2.5* (1.4)
40 ± 8.7 (1.3)
132 ± 7.0 (0.9)
142 ± 3.1 (0.9)
140 ± 1.5 (0.9)
145 ± 6.5 (1.0)
198 ± 12.5 (0.8)
206 ± 11.4 (0.9)
0.75
42 ± 4.0** (1.4)
40 ± 7.5 (1.3)
138 ± 3.6 (0.9)
146 ± 5.1 (1.0)
145 ± 13.2 (0.9)
156 ± 12.4 (1.1)
213 ± 23.3 (0.9)
216 ± 17.1 (1.0)
1.50
50 ± 4.9** (1.7)
49 ± 6.4* (1.6)
156 ± 9.3 (1.1)
146 ± 5.9 (1.0)
155 ± 5.0 (1.0)
154 ± 6.5 (1.1)
246 ± 7.4 (1.0)
232 ± 13.6 (1.1)
2.50
57 ± 5.0**(1.9)
49 ± 4.6* (1.6)
158 ± 3.2 (1.1)
151 ± 7.8 (1.1)
136 ± 4.6 (0.9)
142 ± 7.9 (0.9)
250 ± 8.5 (1.0)
234 ± 11.0 (1.1)
Fraction C (alkaloids)
0
43 ± 2.7
36 ± 2.0
131 ± 1.5
139 ± 2.5
163 ± 4.2
145 ± 9.5
242 ± 9.2
223 ± 11.9
0.12
37 ± 4.0 (0.9)
33 ± 5.0 (0.9)
133 ± 4.5 (1.0)
126 ± 6.6 (0.9)
165 ± 3.6 (1.0)
132 ± 3.1 (0.9)
241 ± 7.1 (0.9)
220 ± 4.5 (0.9)
0.38
43 ± 2.5 (1.0)
36 ± 6.0 (1.0)
146 ± 7.0 (1.1)
136 ± 9.1 (0.9)
178 ± 3.1 (1.1)
142 ± 2.0 (0.9)
256 ± 21.6 (1.1)
224 ± 9.9 (1.0)
0.75
44 ± 1.5 (1.0)
34 ± 11.1 (0.9)
136 ± 8.6 (1.0)
133 ± 4.5 (0.9)
183 ± 4.0 (1.1)
144 ± 4.6 (0.9)
294 ± 12.9 (1.2)
223 ± 14.1 (1.0)
1.50
72 ± 9.7* (1.7)
45 ± 3.1 (1.3)
152 ± 13.1 (1.2)
146 ± 4.0 (1.1)
189 ± 3.5 (1.1)
141 ± 10.0 (0.9)
310 ± 2.0 (1.3)
224 ± 11.2 (1.0)
Control +
608 ± 71.52
663 ± 35.4
711 ± 12.1
761 ± 28.0
914 ± 17.6
886 ± 49.3
1076 ± 57.1
1082 ± 72.9
MeOH: methanolic extract; 0 = negative control (DMSO—100 μl/plate); Control +: Positive control. 4-Nitro-o-phenylenediamine (10.0 μg/plate); 2-anthramine (1.25 μg/plate); Sodium azide (1.25 μg/plate); Mitomycin C (0.5 μg/plate).
*
P < .05, **P < .01 (ANOVA). The values in brackets are MI values.
