Mutagenic Activity of Indigofera truxillensis and I. suffruticosa Aerial Parts
Table 2
Mutagenic activity expressed as the mean and SD of the number of revertants/plate in bacterial strains TA98, TA100, TA97a and TA102 exposed to MeOH extract and fractions A, B and C of I. suffruticosa, at various doses, with (+S9) or without (−S9) metabolic activation.
Treatments mg/plate
Revertants/plate in S. typhimurium strains
TA98
TA97a
TA100
TA102
−S9
+S9
−S9
+S9
−S9
+S9
−S9
+S9
MeOH extract
0
43 ± 2.7
36 ± 2.0
131 ± 1.5
139 ± 2.5
163 ± 4.2
145 ± 9.5
242 ± 9.2
223 ± 11.9
1.25
38 ± 4.6 (0.9)
41 ± 1.2 (1.1)
145 ± 3.1 (1.1)
138 ± 2.1 (0.9)
152 ± 4.0 (0.9)
141 ± 2.1 (0.9)
262 ± 2.5 (1.1)
219 ± 4.9 (0.9)
2.50
43 ± 4.5 (1.0)
40 ± 2.7 (1.1)
145 ± 2.1 (1.1)
142 ± 4.2 (1.0)
151 ± 2.1 (0.9)
147 ± 6.7 (1.0)
244 ± 7.6 (1.0)
225 ± 8.5 (1.0)
3.75
62 ± 2.5** (1.4)
44 ± 3.1 (1.2)
147 ± 4.0 (1.1)
145 ± 2.1 (1.0)
161 ± 2.7 (0.9)
146 ± 7.5 (1.0)
249 ± 6.7 (1.0)
242 ± 5.0 (1.1)
5.00
66 ± 1.5** (1.5)
44 ± 3.1 (1.2)
153 ± 5.0 (1.2)
142 ± 6.4 (1.0)
165 ± 3.1 (1.0)
152 ± 6.2 (1.0)
250 ± 6.1 (1.0)
234 ± 7.9 (1.0)
7.50
76 ± 5.9** (1.8)
45 ± 3.5 (1.2)
159 ± 7.8 (1.2)
151 ± 5.1 (1.0)
156 ± 1.5 (0.9)
149 ± 6.0 (1.0)
253 ± 3.5 (1.0)
237 ± 9.6 (1.1)
Fraction A (glycerolipids)
0
43 ± 2.7
36 ± 2.0
131 ± 1.5
139 ± 2.5
148 ± 2.5
143 ± 5.0
239 ± 16.7
210 ± 11.1
0.12
46 ± 1.7 (1.1)
35 ± 2.0 (0.9)
131 ± 2.1 (1.0)
136 ± 3.5 (0.9)
159 ± 4.2 (1.1)
145 ± 3.0 (1.0)
251 ± 7.0 (1.1)
227 ± 7.6 (1.1)
0.38
47 ± 1.5 (1.1)
38 ± 2.5 (1.1)
134 ± 3.1 (1.0)
135 ± 3.1 (0.9)
160 ± 2.0 (1.1)
147 ± 3.1 (1.0)
260 ± 6.0 (1.1)
235 ± 5.0 (1.1)
0.75
50 ± 1.5 (1.2)
42 ± 1.5 (1.2)
139 ± 5.0 (1.1)
138 ± 1.2 (0.9)
163 ± 3.1 (1.1)
152 ± 2.1 (1.1)
264 ± 8.4 (1.1)
247 ± 5.0 (1.2)
1.50
55 ± 3.6 (1.1)
45 ± 1.6 (1.3)
143 ± 2.5 (1.1)
142 ± 2.1 (1.0)
170 ± 1.5 (1.1)
160 ± 2.0 (1.1)
265 ± 5.0 (1.1)
255 ± 5.0 (1.2)
Fraction B (flavonoids)
0
43 ± 2.7
36 ± 2.0
131 ± 1.5
139 ± 2.5
148 ± 2.5
143 ± 5.0
239 ± 16.7
210 ± 11.1
0.12
45 ± 1.5 (1.0)
42 ± 2.0 (1.2)
142 ± 1.5 (1.0)
136 ± 2.0 (1.0)
149 ± 4.2 (1.0)
136 ± 3.1 (0.9)
244 ± 3.2 (1.0)
219 ± 8.1 (1.0)
0.38
52 ± 1.5* (1.2)
43 ± 4.5 (1.2)
142 ± 1.0 (1.0)
139 ± 1.5 (1.0)
152 ± 3.6 (1.0)
140 ± 2.5 (0.9)
249 ± 2.1 (1.0)
239 ± 6.1 (1.1)
0.75
63 ± 3.1** (1.5)
48 ± 1.2** (1.3)
144 ± 3.1 (1.1)
141 ± 2.1 (1.0)
153 ± 5.5 (1.0)
142 ± 2.1 (0.9)
248 ± 26.2 (1.0)
243 ± 5.0 (1.2)
1.50
81 ± 1.5** (1.9)
54 ± 2.5**(1.5)
149 ± 1.5 (1.1)
144 ± 3.2 (1.0)
158 ± 2.0 (1.1)
143 ± 2.0 (1.0)
272 ± 6.2 (1.1)
264 ± 4.7 (1.3)
Fraction C (alkaloids)
0
43 ± 2.7
36 ± 2.0
131 ± 1.5
139 ± 2.5
148 ± 2.5
143 ± 5.0
239 ± 16.7
210 ± 11.1
0.12
46 ± 1.5 (1.1)
31 ± 1.7 (0.9)
142 ± 2.0 (1.0)
139 ± 1.2 (1.0)
151 ± 2.3 (1.0)
140 ± 2.7 (0.9)
255 ± 4.2 (1.1)
228 ± 5.7 (1.1)
0.38
50 ± 2.0 (1.2)
38 ± 1.5 (1.1)
146 ± 1.2 (1.1)
139 ± 3.1 (1.0)
153 ± 2.5 (1.0)
145 ± 3.0 (1.0)
269 ± 1.2 (1.1)
239 ± 4.2 (1.1)
0.75
69 ± 1.2* (1.6)
40 ± 1.5 (1.1)
152 ± 2.0 (1.0)
143 ± 3.1 (1.0)
165 ± 3.8 (1.1)
149 ± 2.1 (1.0)
278 ± 5.3 (1.2)
243 ± 5.9 (1.2)
1.50
85 ± 1.5**(1.9)
47 ± 1.2 (1.3)
152 ± 4.4 (1.2)
143 ± 1.5 (1.1)
180 ± 2.0 (1.2)
154 ± 2.5 (1.1)
306 ± 7.2 (1.3)
255 ± 4.7 (1.2)
Control +
466 ± 34.1
607 ± 30.3
703 ± 24.7
750 ± 55.7
955 ± 33.0
797 ± 32.1
1158 ± 65.8
1158 ± 49.4
MeOH: methanolic extract; 0 = negative control (DMSO—100 μl/plate); Control +: positive control. 4-Nitro-o-phenylenediamine (5.0 μg/plate); 2-anthramine (1.25 μg/plate); Sodium azide (1.25 μg/plate); Mitomycin C (0.5 μg/plate).
*
P < .05, **P < .01 (ANOVA). The values in brackets are MI values.
