Mutagenic Activity of Indigofera truxillensis and I. suffruticosa Aerial Parts

Table 3

Mutagenic activity expressed as the mean and SD of the number of revertants/plate in bacterial strain TA98 exposed to the compounds isolated from methanolic extract of I. truxillensis and I. suffruticosa at various doses, with (+S9) or without (−S9) metabolic activation.

Treatment (mg/plate)	TA98 (−S9)	TA98 (+S9)
Positive control	867 ± 1.4	991 ± 10.1
Negative control	30 ± 1.5	36 ± 4.0
Alkaloids
Indigo
0.125	38 ± 0.6 (1.3)	44 ± 2.7* (1.2)
0.25	42 ± 3.2 (1.4)	86 ± 5.3** (2.4)
0.50	45 ± 1.5 (1.5)	192 ± 2.5** (5.3)
0.75	50 ± 4.0* (1.7)	229 ± 3.6** (6.7)
1.00	57 ± 4.2** (1.9)	276 ± 5.3** (7.7)
Indirubin
0.125	46 ± 2.7 (1.5)	36 ± 2.0 (1.0)
0.25	58 ± 3.8** (1.9)	36 ± 4.0 (1.0)
0.50	58 ± 3.0** (1.9)	38 ± 2.5 (1.1)
0.75	63 ± 1.7** (2.1)	43 ± 4.0 (1.2)
1.00	63 ± 3.6** (2.1)	46 ± 3.6 (1.3)

Negative control: DMSO (100 μl/plate); Positive control: −S9: 4-nitro-o-phenylenediamine (10.0 μg/plate); +S9: 2-anthramine (1.25 μg/plate).
* P < .05, **P < .01 (ANOVA). The values in brackets are MI values.