Neuron Regeneration and Proliferation Effects of Danshen and Tanshinone IIA
Figure 2
Proliferative effects of danshen and tanshinone IIA on RSC96 cell viability and migration. RSC cells were incubated with different doses of danshen and tanshinone IIA as indicated. Cell viability was measured using MTT assay (a), and western blotting analysis (b); cell migration was measured using wound healing analysis (c). The procedures are described in the materials and methods section. The wound healing data were quantified by measuring the number of cells that had advanced into the cell-free space (d). Data are shown as the mean of three independent experiments ± SE. *, **, and *** denote significant differences between experimental and control values, with , , and , respectively. α-Tubulin was used as a loading control. PCNA: proliferating cell nuclear antigen.
