Antioxidant and Preventive Effects of Extract from Nymphaea candida Flower on In Vitro Immunological Liver Injury of Rat Primary Hepatocyte Cultures

Table 6

Effect of NCE on supernatant ALT levels in immunologic injury in primary cultured rat hepatocytes in vitro.

Group	Dose	ALT (U/L)
	𝜇g/mL	3 hours	6 hours	12 hours	24 hours
Control	—	3.16 ± 0.58	3.68 ± 0.42	4.68 ± 0.31	3.65 ± 0.53
BCG + LPS	—	5.69 ± 0.91^a	7.68 ± 0.74^a	9.43 ± 1.23^a	6.58 ± 0.83^a
BCG + LPS + Grz	5	5.12 ± 1.36	4.08 ± 1.00^b	6.61 ± 1.01^b	3.11 ± 0.59^b
	10	3.62 ± 0.73^b,e	3.29 ± 1.05^b	5.14 ± 0.85^b,e	3.80 ± 1.42^b
	20	3.98 ± 0.64^b	4.80 ± 1.65^b	4.87 ± 0.80^b,d	3.20 ± 1.24^b
BCG + LPS + NCE	5	5.71 ± 1.10	3.95 ± 0.53^b	6.06 ± 0.70^b	3.52 ± 0.37^b
	20	4.25 ± 1.17^c	3.77 ± 0.63^b	4.88 ± 0.30^b	3.21 ± 0.49^b
	80	4.71 ± 1.23	2.53 ± 0.97^b	4.07 ± 0.86^b	3.29 ± 0.98^b

Values are the mean ± SD, n = 6. ^a𝑃<.01 compared with control group; ^b𝑃<.01 and ^c𝑃<.05 compared with BCG + LPS group; ^d𝑃<.01 and ^e𝑃<.01 compared with BCG + LPS + Grz group (5 𝜇g/mL).