Antiosteoporotic Activity of Dioscorea alata L. cv. Phyto through Driving Mesenchymal Stem Cells Differentiation for Bone Formation

Figure 2

Dispo85E stimulates osteoblastogenesis in bone-marrow-derived cells. Primary bone-marrow-derived cells were cultured with osteogenic medium and treated with Dispo85E at the indicated concentrations. At PID 6, (a) cellular alkaline phosphatase (ALP) activity and (b) the effect of Dispo85E on cell viability were measured (𝑛=4). (c) At PID 16, cells were stained with alizarin red S, and (d) the mineralized area was quantified by extracting alizarin red S staining with 10% cetylpyridinium chloride (CPC) and measuring the OD of the extract at 550 nm (𝑛=3). **Indicates significant difference from control group at 𝑃<.01; *Indicates significance at 𝑃<.05.

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