Research Article

Apoptotic Cell Death and Inhibition of Wnt/β-Catenin Signaling Pathway in Human Colon Cancer Cells by an Active Fraction (HS7) from Taiwanofungus camphoratus

Figure 4

HS7 regulated expression of specific proteins in HT29 cells involved in apoptosis. (a) Protein expression of Bax and Bcl-2 and Bax/Bcl-2 ratio were shown. HT-29 cells were exposed to 2.5–25 μg/mL of HS7 for 48 h. Bax and Bcl-2 expression was determined by Western blot. Relative changes in Bcl-2 and Bax protein bands were measured using densitometric analysis. (b) HS7 induced caspase-3 activation and cleaved PARP in HT29 cells. Typical results from 3 independent experiments were shown.
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