Apoptotic Cell Death and Inhibition of Wnt/β-Catenin Signaling Pathway in Human Colon Cancer Cells by an Active Fraction (HS7) from Taiwanofungus camphoratus
Figure 6
HS7 dose-dependently decreased the binding of Tcf complex to DNA and downregulated the protein levels of active β-catenin, Tcf-4, and p-GSK3α/β. (a) HT-29 cells were treated with HS7 at the indicated concentrations for 48 h, and nuclear extracts were isolated. The electrophoretic-mobility gel shift assay (EMSA) was performed with 5 μg nuclear extracts. (b) HT-29 cells were treated with HS7 (0–25 μg/mL) for 48 h, the changes of active β-catenin, Tcf-4, and p-GSK3α/β protein levels were then analyzed by Western blot. β-actin was used as internal control. All pictures are representative of two independent experiments.
