Apoptotic Cell Death and Inhibition of Wnt/β-Catenin Signaling Pathway in Human Colon Cancer Cells by an Active Fraction (HS7) from Taiwanofungus camphoratus
Figure 7
Effect of HS7 on cellular localization of β-catenin in HT-29 cells was shown. Cellular localization of β-catenin was evaluated by indirect immunofluorescence using a monoclonal antibody that labeled β-catenin. Immunofluorescence labeling of β-catenin in cells treated with vehicle (a) or 10 μg/mL HS7 (c) for 48 h were shown (red). Nuclei in cells treated with vehicle (b) or 10 μg/mL HS7 (d) for 48 h were counterstained with 4′, 6-diamidino-2-phenylindole (blue). Merged images show that the β-catenin protein located at cell-cell contacts in HS7-treated cells was significantly increased as compared to control (e and f). Magnification for each representative picture: 200x (from 3 separate experiments).
