Flourensia cernua: Hexane Extracts a Very Active Mycobactericidal Fraction from an Inactive Leaf Decoction against Pansensitive and Panresistant Mycobacterium tuberculosis

Table 1

Yield and mycobactericidal activity of crude extracts of Flourensia cernua leaf obtained with different extraction methods.

Extraction method	Solvent	Yield^a	MBC^b (μg/mL)		Resulting extract
			SMtb^c	RMtb^d
DC	W	24.4	>100	>100	A
StMC	EtOH	25.5	100	50	B
SXH	EtOH	31.3	100	50	C
UsMC	EtOH	22.7	100	50	D
SkMC	EtOH	30.9	100	50	E

^aRecovering percentage with respect to 15 g dry ground leaves (%w/w);  ^bmycobactericidal activity MBC was expressed as the minimal bactericidal activity (MBC) that killed 100% of mycobacteria. ^cSMtb M. tuberculosis H37Rv strain sensitive to all five fist-line antituberculosis drugs; ^dRMtbCIBIN/UMF15:99, resistant to the above drugs. DC: decoction, StMC: still maceration, SXH: Soxhlet extraction, UsMC: ultrasound-assisted maceration, and SkMC: shake-assisted maceration; W: water; EtOH: ethanol. Rifampin showed an MBC=0.062 and 100 μg/mL and ofloxacin MBC=0.125 and 0.250 μg/mL versus SMtb and RMtb, respectively.