Evidence-Based Complementary and Alternative Medicine

Research Article

Flourensia cernua: Hexane Extracts a Very Active Mycobactericidal Fraction from an Inactive Leaf Decoction against Pansensitive and Panresistant Mycobacterium tuberculosis

Table 4

Absolute mycobactericidal activity against SMtb in F. cernua leaf preparationsa.
Extract (solvent)Fraction (solvent)Extraction or fractionation methodAMAb (MBU) AMAcNAMAd
A (W)DCNDNQNQ
A1 (n-Hex)LLP98,088
A2 (EtOAc)LLP73,200
A3 (n-BuOH)LLP6,478177,7661.0
B (EtOH)StMC38,2500.22
B1 (n-Hex)LLP37,791
B2 (EtOAc)LLP16,333
B3 (n-BuOH)LLP2,10456,2280.32
C (EtOH)SXH46,9500.26
C1 (n-Hex)LLP64,228
C2 (EtOAc)LLP35,588
C3 (n-BuOH)LLP4,977104,7930.59
D (EtOH)UsMC34,0500.19
D1 (n-Hex)LLP34,459
D2 (EtOAc)LLP17,570
D3 (n-BuOH)LLP3,84855,8770.31
E (EtOH)SkMC46,3500.26
E1 (n-Hex)LLP42,086
E2 (EtOAc)LLPND
(E3) (n-BuOH)LLP3,10645,1920.25
F (n-Hex)StMC27,000
G (EtOAc)StMC14,400
H (EtOH)StMC24,90066,3000.37
I (n-Hex)SXH28,500
J (EtOAc)SXH12,300
K (EtOH)SXH18,15058,9500.33
L (n-Hex)UsMC34,500
M (EtOAc)UsMC5,850
N (EtOH)UsMC8,25048,6000.27
O (n-Hex)SkMC37,500
P (EtOAc)SkMC8,250
Q(EtOH)SkMC25,65071,4000.40
aObtaining of extracts and their fractions as well as their correspondent yields and MBC is described in the footnotes of Tables 1–3. bAMA means absolute mycobactericidal activity and is expressed as the number of mycobactericidal units (MBU) contained in the total mass of solids from an extract or fraction. One MBU is equivalent to the minimal mycobactericidal concentration expressed in g. The total content of solids in all extracts was calculated from yields of each extract or fraction, considering that all of these were obtained from portions of 15 g leaf powder (Tables 1–3). The solid mass of fractions was calculated considering the total solids of their correspondent extract. All calculations were performed using the equations and definitions described in Section 2. cΣAMA is the sum of MUs in all fractions derived from decoction or ethanol extracts, or the sum of extracts that came from the same portion of leaf powder and obtained with the same extraction method but with a different solvent (n-Hex, EtOAc, and EtOH). dNAMA means normalized absolute mycobactericidal activity. 1.00 = Sum of AMA in the decoction fractions A1–A3.  ND means not detected and NQ not quantifiable.